Anticorps Monoclonal anti-TLR8

TLR8 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B6A4

N° de cat : 67317-1-PBS

Synonymes

CD288, TLR8, toll like receptor 8

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Galerie de données de validation

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  WB analysis using 67317-1-Ig (same clone as 67317-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 67317-1-Ig (same clone as 67317-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 67317-1-Ig (TLR8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67317-1-Ig (TLR8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 67317-1-Ig (same clone as 67317-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TLR8 antibody (67317-1-Ig, Clone: 3B6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67317-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67317-1-PBS cible TLR8 dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TLR8 Protéine recombinante Ag16566
• Nom complet: toll-like receptor 8
• Masse moléculaire calculée: 1041 aa, 120 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC101075
• Symbole du gène: TLR8
• Identification du gène (NCBI): 51311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Toll-like receptors (TLRs) are a group of pathogen recognition receptors (PRRs) that can be divided into two subfamilies depending on their subcellular localization(PMID: 32268138). Toll-like receptor 8 (TLR8), a sensor for pathogen-derived single-stranded RNA (ssRNA), binds to uridine (Uri) and ssRNA to induce defense responses (PMID:30535046). TLR8 proteins were expressed in spleen, epididymal sperm, testicular sperm cells, and Leydig cells but were weak in Sertoli cells. It can identify two isoforms,120 and 122 kDa.

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