Anticorps Polyclonal de lapin anti-TM7SF2

TM7SF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12033-1-AP

Synonymes

C14SR, C-14 sterol reductase, C 14 sterol reductase, Another new gene 1 protein, 3-beta-hydroxysterol Delta

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Galerie de données de validation

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  WB analysis of human lung using 12033-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 12033-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12033-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12033-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12033-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12033-1-AP (TM7SF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12033-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12033-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12033-1-AP (TM7SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 12033-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using TM7SF2 antibody (12033-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire humain, cellules BxPC-3, tissu cardiaque humain, tissu rénal humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

12033-1-AP cible TM7SF2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TM7SF2 Protéine recombinante Ag2637
• Nom complet: transmembrane 7 superfamily member 2
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC012857
• Symbole du gène: TM7SF2
• Identification du gène (NCBI): 7108
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	PLoS One; Hidden disease susceptibility and sexual dimorphism in the heterozygous knockout of cyp51 from cholesterol synthesis.; Authors - Monika Lewinska
human	WB,IHC	Curr Oncol; TM7SF2 as a Potential Biomarker in Colorectal Cancer: Implications for Metastasis; Authors - Inpyo Hong
human	WB	J Ethnopharmacol; Tylophora yunnanensis extract inhibits cholesterol biosynthesis to suppress triple negative breast cancer; Authors - Zhi-Hui Feng

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