Anticorps Polyclonal de lapin anti-TMP21

• Phare
• Validé par KD/KO

TMP21 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(9)

Avis (2)

N° de cat : 15199-1-AP

Synonymes

TMED10, 21 kDa transmembrane-trafficking protein, p23, p24 family protein delta-1, p24delta1

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Galerie de données de validation

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  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15199-1-AP (TMP21 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 15199-1-AP

  IP result of anti-TMP21 (IP:15199-1-AP, 4ug; Detection:15199-1-AP 1:3000) with BxPC-3 cells lysate 2080 ug.

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15199-1-AP (TMP21 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 15199-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15199-1-AP (TMP21 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HEK-293T, cellules MG U-87, cellules Neuro-2a, cellules NIH/3T3, cellules PC-3, cellules Raji, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IP: cellules BxPC-3,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15199-1-AP cible TMP21 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMP21 Protéine recombinante Ag7347
• Nom complet: transmembrane emp24-like trafficking protein 10 (yeast)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC001825
• Symbole du gène: TMED10
• Identification du gène (NCBI): 10972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse,human	WB,IF	Cell; A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.; Authors - Min Zhang ; KO Validated
human	WB	Nat Cell Biol; A dual role of ERGIC-localized Rabs in TMED10-mediated unconventional protein secretion; Authors - Yuxin Sun
human	WB	Cell Res; A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.; Authors - Shulin Li
human	WB	Brain Behav Immun; Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis; Authors - Mengya Jiao
human	CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun
human	WB,IF	BMC Cancer; The circular RNA CDR1as regulate cell proliferation via TMED2 and TMED10.; Authors - Xue Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

pitna (Verified Customer) (12-19-2019)

Tmp21, a subunit of the p24 complexTo reduce non-specific binding, blots were incubated in LiCor Buffer for an hour at room temperature. Representative images from Western blots of total homogenate and ER fractions

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: Postmortem human brain : dorsolateral prefrontal cortex (DLPFC)

TMP21 Antibody Western Blot, validation (1:1,000 dilution) in Postmortem human brain : dorsolateral prefrontal cortex (DLPFC) (Cat no:15199-1-AP)

FH

Boyan (Verified Customer) (01-25-2019)

Good. By IF, PFA fixation, it labels the protein at the Golgi.

• Applications: Immunofluorescence,

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