Anticorps Monoclonal anti-TMP21

TMP21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

3B11F9

N° de cat : 67876-1-Ig

Synonymes

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

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Galerie de données de validation

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  WB analysis using 67876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67876-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67876-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67876-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMP21 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 67876-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug TMP21 Monoclonal antibody (67876-1-Ig, Clone:3B11F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules Jurkat, cellules Raji, tissu hépatique de porc, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67876-1-Ig cible TMP21 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMP21 Protéine recombinante Ag7516
• Nom complet: transmembrane emp24-like trafficking protein 10 (yeast)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC001825
• Symbole du gène: TMED10
• Identification du gène (NCBI): 10972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Autophagy-independent role of ATG9A vesicles as carriers for galectin-9 secretion; Authors - Wenting Zhang ; KD Validated

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