Anticorps Monoclonal anti-TMP21

TMP21 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3B11F9

N° de cat : 67876-1-PBS

Synonymes

TMED10, Protein TMED10, p24delta1, p24 family protein delta-1, p23

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Galerie de données de validation

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  WB analysis using 67876-1-Ig (same clone as 67876-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67876-1-Ig (TMP21 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67876-1-Ig (TMP21 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TMP21 antibody (67876-1-Ig, Clone: 3B11F9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67876-1-Ig (same clone as 67876-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug TMP21 Monoclonal antibody (67876-1-Ig, Clone:3B11F9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67876-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67876-1-PBS cible TMP21 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMP21 Protéine recombinante Ag7516
• Nom complet: transmembrane emp24-like trafficking protein 10 (yeast)
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC001825
• Symbole du gène: TMED10
• Identification du gène (NCBI): 10972
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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