Anticorps Polyclonal de lapin anti-TMED3

• Phare
• Validé par KD/KO

TMED3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 21902-1-AP

Synonymes

Transmembrane emp24 domain-containing protein 3, p26, p24gamma4, p24 family protein gamma-4, Membrane protein p24B

---

Galerie de données de validation

• 

  WB analysis using 21902-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 21902-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21902-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 21902-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 21902-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21902-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21902-1-AP (TMED3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 21902-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21902-1-AP (TMED3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HT-1080, cellules MCF-7, tissu cutané de souris, tissu d'intestin grêle de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below

Informations sur le produit

21902-1-AP cible TMED3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMED3 Protéine recombinante Ag14032
• Nom complet: transmembrane emp24 protein transport domain containing 3
• Masse moléculaire calculée: 217 aa, 25 kDa
• Poids moléculaire observé: 21-25 kDa
• Numéro d’acquisition GenBank: BC000027
• Symbole du gène: TMED3
• Identification du gène (NCBI): 23423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
human	WB	J Biol Chem; Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.; Authors - Chengfeng Merriman
human	WB	Toxicol Appl Pharmacol; TMED3 exerts a protumor function in non-small cell lung cancer by enhancing the Wnt/β-catenin pathway via regulation of AKT.; Authors - Danjie Zhang ; KD Validated
human	WB	Onco Targets Ther; TMED3 Promotes Proliferation and Migration in Breast Cancer Cells by Activating Wnt/β-Catenin Signaling.; Authors - Xiumei Zhang ; KD Validated
human	WB	Ann Transl Med; Screening of the novel immune-suppressive biomarkers of TMED family and whether knockdown of TMED2/3/4/9 inhibits cell migration and invasion in breast cancer; Authors - Zheng Fang ; KD Validated
human	WB	J Cancer Res Clin Oncol; Analysis and Validation of TMED3 correlates with poor prognosis and tumor immune infiltration of glioma; Authors - Gang Liao

{{ptg:RelatedPrimaryAntibodies}}