Anticorps Monoclonal anti-TMEM111

• Phare
• Validé par KD/KO

TMEM111 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F8G5

N° de cat : 67205-1-PBS

Synonymes

EMC3, 2F8G5, POB

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Galerie de données de validation

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  WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67205-1-Ig (same clone as 67205-1-PBS)

  WB result of TMEM111 antibody (67205-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM111 transfected HEK-293 cells. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67205-1-Ig (same clone as 67205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 67205-1-Ig (same clone as 67205-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 67205-1-Ig (same clone as 67205-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 67205-1-Ig (same clone as 67205-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67205-1-Ig (same clone as 67205-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67205-1-Ig (TMEM111 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 67205-1-Ig (same clone as 67205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 67205-1-Ig (TMEM111 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67205-1-Ig (same clone as 67205-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using TMEM111 antibody (67205-1-Ig, Clone: 2F8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67205-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67205-1-PBS cible TMEM111 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMEM111 Protéine recombinante Ag18365
• Nom complet: transmembrane protein 111
• Masse moléculaire calculée: 261 aa, 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC022807
• Symbole du gène: TMEM111
• Identification du gène (NCBI): 55831
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMEM111,also names as EMC3, is part of the endoplasmic reticulum-associated secretory pathway (PMID: 19797678). TMEM111 was required for murine pulmonary surfactant synthesis and lung function at birth (PMID: 29083321). It can be detect a band of 30 kDa.

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