Anticorps Polyclonal de lapin anti-TMEM119

TMEM119 Polyclonal Antibody for IHC, IF-P, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

N° de cat : 27585-1-PBS

Synonymes

UNQ731/PRO1415, transmembrane protein 119, Osteoblast induction factor, OBIF

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Galerie de données de validation

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  IHC staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27585-1-AP (TMEM119 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 27585-1-AP (same clone as 27585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 27585-1-AP (same clone as 27585-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TMEM119 antibody (27585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 27585-1-AP (same clone as 27585-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug TMEM119 Polyclonal antibody (27585-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 27585-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27585-1-PBS cible TMEM119 dans les applications de IHC, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM119 Protéine recombinante Ag26269
• Nom complet: transmembrane protein 119
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_181724
• Symbole du gène: TMEM119
• Identification du gène (NCBI): 338773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMEM119 immunohistochemistry might provide a useful tool for investigating the biology and pathology of human microglia(PMID: 26250788). Microglia can be detected clearly using Catalog#27585-1-AP.

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