Anticorps Polyclonal de lapin anti-TMEM165

TMEM165 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20485-1-PBS

Synonymes

Putative divalent cation/proton antiporter TMEM165, TMPT27, TPARL, Transmembrane protein 165, Transmembrane protein PT27

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Galerie de données de validation

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  WB analysis of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20485-1-AP (TMEM165 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  IP result of anti-TMEM165 (IP:20485-1-AP, 4ug; Detection:20485-1-AP 1:1000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 20485-1-AP (same clone as 20485-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMEM165 antibody (20485-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594-Phalloidin (red). This data was developed using the same antibody clone with 20485-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20485-1-PBS cible TMEM165 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM165 Protéine recombinante Ag14362
• Nom complet: transmembrane protein 165
• Masse moléculaire calculée: 324 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC003545
• Symbole du gène: TMEM165
• Identification du gène (NCBI): 55858
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Thiopurine methyltransferase or thiopurine S-methyltransferase (TPMT), a member of methyltransferase superfamily, is an enzyme involved in the normal metabolic inactivation of thiopurine drugs such as azathioprine, 6-mercaptopurine and 6-thioguanine. These drugs are generally used as immunosupressants or cytotoxic drugs and are prescribed for a variety of clinical conditions including leukemia, autoimmune disease and organ transplantation. Defects in the TPMT gene leads to decreased methylation and decreased inactivation of 6MP leading to enhanced bone marrow toxicity which may cause myelosuppression, anemia, bleeding tendency, leukopenia and infection.

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