Anticorps Monoclonal anti-TMEM173/STING

• Phare
• Validé par KD/KO

TMEM173/STING Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1F1E1

N° de cat : 66680-1-PBS

Synonymes

STING, NET23, MPYS, MITA, ERIS

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Galerie de données de validation

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  WB analysis of THP-1 using 66680-1-Ig (same clone as 66680-1-PBS)

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  WB analysis using 66680-1-Ig (same clone as 66680-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66680-1-Ig (same clone as 66680-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66680-1-Ig (same clone as 66680-1-PBS)

  WB result of TMEM173/STING antibody (66680-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66680-1-Ig (same clone as 66680-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66680-1-Ig (TMEM173 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66680-1-Ig (same clone as 66680-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66680-1-Ig (TMEM173/STING antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 66680-1-Ig (same clone as 66680-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using TMEM173/STING antibody (66680-1-Ig, Clone: 1F1E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66680-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66680-1-PBS cible TMEM173/STING dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMEM173/STING Protéine recombinante Ag20363
• Nom complet: transmembrane protein 173
• Masse moléculaire calculée: 379 aa, 42 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC047779
• Symbole du gène: STING
• Identification du gène (NCBI): 340061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

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