Anticorps Polyclonal de lapin anti-TMEM173/STING

• Phare
• Validé par KD/KO

TMEM173/STING Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, IF-P, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications(2)

N° de cat : CL594-19851

Synonymes

STING, NET23, MPYS, MITA, ERIS

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Galerie de données de validation

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  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  IF Staining of HT-29 using CL594-19851

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  IF Staining of HepG2 using CL594-19851

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 TMEM173/STING antibody (CL594-19851) at dilution of 1:200.

• 

  FC experiment of THP-1 using CL594-19851

  1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TMEM173/STING (CL594-19851) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF-P: tissu d'amygdalite humain,
• Résultats positifs en IF/ICC: cellules HT-29, cellules HepG2, tissu d'amygdalite humain
• Résultats positifs en FC (Intra): cellules THP-1,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

CL594-19851 cible TMEM173/STING dans les applications de IF/ICC, IF-P, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM173/STING Protéine recombinante Ag13921
• Nom complet: transmembrane protein 173
• Masse moléculaire calculée: 379 aa, 42 kDa
• Poids moléculaire observé: 35-40 kDa, 80 kDa
• Numéro d’acquisition GenBank: BC047779
• Symbole du gène: STING
• Identification du gène (NCBI): 340061
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines.

Publications

Species	Application	Title
mouse	IF	J Neuroinflammation; The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage; Authors - Xingfei Zhu
mouse	FC	Mol Pharm; STING-Targeted PET Imaging for Specific Detection and Therapeutic Monitoring of Myocarditis; Authors - Zhou Ye

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