Anticorps Recombinant de lapin anti-TMEM175

• Phare
• Validé par KD/KO

TMEM175 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241567B9

N° de cat : 84554-1-PBS

Synonymes

241567B9, hTMEM175, Transmembrane protein 175

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Galerie de données de validation

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  WB analysis using 84554-1-RR (same clone as 84554-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84554-1-RR (TMEM175 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84554-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 84554-1-RR (same clone as 84554-1-PBS)

  WB result of TMEM175 antibody (84554-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells. This data was developed using the same antibody clone with 84554-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84554-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84554-1-RR against Human TMEM175 were performed. The affinity constant is 5.17 nM.

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Informations sur le produit

84554-1-PBS cible TMEM175 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TMEM175 Protéine recombinante Ag13890
• Nom complet: transmembrane protein 175
• Masse moléculaire calculée: 504 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC005158
• Symbole du gène: TMEM175
• Identification du gène (NCBI): 84286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

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