Anticorps Polyclonal de lapin anti-TMEM41B

• Phare
• Validé par KD/KO

TMEM41B Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 29270-1-AP

Synonymes

KIAA0033, TMEM41B, transmembrane protein 41B

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Galerie de données de validation

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  WB analysis of HeLa using 29270-1-AP

  WB result of TMEM41B antibody (29270-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM41B transfected HeLa cells.

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  WB analysis using 29270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29270-1-AP (TMEM41B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 29270-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 29270-1-AP (TMEM41B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa
• Résultats positifs en IHC: tissu pulmonaire de souris, tissu pulmonaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

29270-1-AP cible TMEM41B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM41B Protéine recombinante Ag30810
• Nom complet: transmembrane protein 41B
• Poids moléculaire observé: 22-28 kDa
• Numéro d’acquisition GenBank: BC035034
• Symbole du gène: TMEM41B
• Identification du gène (NCBI): 440026
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane protein 41B (TMEM41B), also known as Stasimon, is a major target of survival motor neuron (SMN)-dependent U12 splicing and has been identified as a protein required for motor circuit function (PMID: 23063131). TMEM41B localizes to the endoplasmic reticulum. It is involved in autophagosome biogenesis and lipid mobilization (PMID: 30126924). TMEM41B has been shown to be essential for mouse embryonic development (PMID: 30352685). Additionally, genome-scale CRISPR knockout screens reveal that TMEM41B is required for infection of flavivirus and coronavirus, including SARS-CoV-2 (PMID: 33052348; 33052332). Alternative splicing results in three transcript variants encoding distinct isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Cell Metab; TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis.; Authors - Dong Huang ; KO Validated
mouse	IHC	Biomedicines; Comparative Proteomic Analysis of tPVAT during Ang II Infusion.; Authors - Xiuying Liang
human	WB	STAR Protoc; In vitro and in vivo assay of the ER lipid scramblase TMEM41B.; Authors - Lingzhi Wu ; KO Validated
mouse,pig	WB,IF	J Virol; TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication; Authors - Xiu-Qing Li ; KD Validated
human	WB	Nature; PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection; Authors - Dijin Xu