Anticorps Polyclonal de lapin anti-TMEM50B

TMEM50B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15941-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse testis using 15941-1-AP (same clone as 15941-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 15941-1-AP (same clone as 15941-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 15941-1-AP (same clone as 15941-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15941-1-AP (TMEM50B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15941-1-AP (same clone as 15941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15941-1-AP (same clone as 15941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15941-1-AP (TMEM50B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15941-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15941-1-PBS cible TMEM50B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM50B Protéine recombinante Ag8220
• Nom complet: transmembrane protein 50B
• Masse moléculaire calculée: 15 kDa
• Poids moléculaire observé: 14 kDa, 30 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC000569
• Symbole du gène: TMEM50B
• Identification du gène (NCBI): 757
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMEM50B, originally referred to as C21orf4, is a predicted transmembrane protein, estimated to contain four transmembrane-spanning domains with both the carboxy- and amino-terminals being cytoplasmic (PMID: 10077530; 18541381). Human TMEM50B shares sequence homologies of around 90-100% with mouse and rat Tmem50b. In a Down syndrome mouse model, Ts1Cje, Tmem50b is shown to be one of the few genes significantly over-expressed during cerebellar development (PMID: 15590701). It has been reported that rodent Tmem50b is a developmentally-regulated intracellular ER and Golgi apparatus membrane protein that may prove important for correct brain development through functions associated with precursor cells and glia (PMID: 18541381). TMEM50B can exist as a dimer and a trimer conformation (PMID: 18541381).

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