Anticorps Recombinant de lapin anti-TMEM53

TMEM53 Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240511C3

N° de cat : 83554-3-PBS

Synonymes

Transmembrane protein 53, Nuclear envelope transmembrane protein 4, NET4, 240511C3

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Galerie de données de validation

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  IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using TMEM53 antibody (83554-3-RR, Clone: 240511C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83554-3-RR (same clone as 83554-3-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug TMEM53 Recombinant antibody (83554-3-RR, Clone:240511C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83554-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83554-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83554-3-RR against Human TMEM53 were performed. The affinity constant is 44.5 nM.

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Informations sur le produit

83554-3-PBS cible TMEM53 dans les applications de IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TMEM53 Protéine recombinante Ag21321
• Nom complet: transmembrane protein 53
• Masse moléculaire calculée: 277 aa, 32 kDa
• Numéro d’acquisition GenBank: BC064520
• Symbole du gène: TMEM53
• Identification du gène (NCBI): 79639
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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