Anticorps Polyclonal de lapin anti-TMEM65

• Phare
• Validé par KD/KO

TMEM65 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21913-1-PBS

Synonymes

Transmembrane protein 65

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Galerie de données de validation

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  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  WB analysis using 21913-1-AP (same clone as 21913-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21913-1-AP (TMEM65 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21913-1-AP (same clone as 21913-1-PBS)

  WB result of TMEM65 antibody (21913-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM65 transfected HEK-293 cells. This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 21913-1-AP (same clone as 21913-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21913-1-AP (TMEM65 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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  IF Staining of Huh-7 using 21913-1-AP (same clone as 21913-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Huh-7 cells using TMEM65 antibody (21913-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21913-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21913-1-PBS cible TMEM65 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMEM65 Protéine recombinante Ag15509
• Nom complet: transmembrane protein 65
• Masse moléculaire calculée: 240 aa, 26 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC032396
• Symbole du gène: TMEM65
• Identification du gène (NCBI): 157378
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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