Anticorps Recombinant de lapin anti-FDX1

• Phare
• Validé par KD/KO

FDX1 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230196E9

N° de cat : 82957-2-PBS

Synonymes

Ferredoxin-1, FDX, ADX, 230196E9

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Galerie de données de validation

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  WB analysis using 82957-2-RR (same clone as 82957-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 82957-2-RR (same clone as 82957-2-PBS)

  WB result of FDX1 antibody (82957-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  WB analysis using 82957-2-RR (same clone as 82957-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82957-2-RR (FDX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82957-2-RR (same clone as 82957-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82957-2-RR (FDX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82957-2-RR (same clone as 82957-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82957-2-RR (FDX1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82957-2-RR (same clone as 82957-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (82957-2-RR, Clone: 230196E9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 82957-2-RR (same clone as 82957-2-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.4 ug FDX1 Recombinant antibody (82957-2-RR, Clone:230196E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82957-2-RR (same clone as 82957-2-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug FDX1 Recombinant antibody (82957-2-RR, Clone:230196E9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), or 0.25 ug FDX1 Recombinant antibody (82957-2-RR, Clone: 230196E9) (green). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82957-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82957-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82957-2-RR against Human FDX1 were performed. The affinity constant is 17.3 nM.

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Informations sur le produit

82957-2-PBS cible FDX1 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: FDX1 Protéine recombinante Ag3301
• Nom complet: ferredoxin 1
• Masse moléculaire calculée: 184 aa, 19 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC017063
• Symbole du gène: FDX1
• Identification du gène (NCBI): 2230
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

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