Anticorps Monoclonal anti-TMF1

• Phare
• Validé par KD/KO

TMF1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G12F2

N° de cat : 67505-1-PBS

Synonymes

Androgen receptor-associated protein of 160 kDa, ARA160, TATA element modulatory factor, TMF

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Galerie de données de validation

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  WB analysis using 67505-1-Ig (same clone as 67505-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67505-1-Ig (TMF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67505-1-Ig (same clone as 67505-1-PBS)

  WB result of TMF1 antibody (67505-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMF1 transfected HeLa cells. This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67505-1-Ig (TMF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67505-1-Ig (same clone as 67505-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TMF1 antibody (67505-1-Ig, Clone: 3G12F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67505-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67505-1-PBS cible TMF1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMF1 Protéine recombinante Ag17666
• Nom complet: TATA element modulatory factor 1
• Masse moléculaire calculée: 1093 aa, 123 kDa
• Poids moléculaire observé: 150-160 kDa
• Numéro d’acquisition GenBank: BC126123
• Symbole du gène: TMF1
• Identification du gène (NCBI): 7110
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMF1, also named as ARA160, is a potential coactivator of the androgen receptor. TMF1 mediates STAT3 degradation. TMF1 plays critical roles in two RAB6-dependent retrograde transport processes: one from endosomes to the Golgi and the other from the Golgi to the ER. TMF1 binds the HIV-1 TATA element and inhibits transcriptional activation by the TATA-binding protein (TBP). The antibody is specific to TMF1. TMF1 can be detected as 150-160 kDa after posttranslational modification.

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