Anticorps Recombinant de lapin anti-TMIGD2/CD28H

TMIGD2/CD28H Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241807C12

N° de cat : 84727-5-PBS

Synonymes

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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Galerie de données de validation

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  WB analysis using 84727-5-RR (same clone as 84727-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84727-5-PBS in a different storage buffer formulation.

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  WB analysis of pig thymus using 84727-5-RR (same clone as 84727-5-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84727-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84727-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

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Informations sur le produit

84727-5-PBS cible TMIGD2/CD28H dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: transmembrane and immunoglobulin domain containing 2
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC015655
• Symbole du gène: TMIGD2
• Identification du gène (NCBI): 126259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).

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