Anticorps Recombinant de lapin anti-TMIGD2/CD28H

TMIGD2/CD28H Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241807C12

N° de cat : 84727-5-RR

Synonymes

TMIGD2/CD28, Immunoglobulin and proline-rich receptor 1, IGPR-1, IGPR1, CD28H

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Galerie de données de validation

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  WB analysis using 84727-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig thymus using 84727-5-RR

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 84727-5-RR (TMIGD2/CD28H antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84727-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84727-5-RR against Human TMIGD2/CD28H were performed. The affinity constant is 1.23 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules MOLT-4, tissu de thymus de porc

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84727-5-RR cible TMIGD2/CD28H dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: transmembrane and immunoglobulin domain containing 2
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 48 kDa
• Numéro d’acquisition GenBank: BC015655
• Symbole du gène: TMIGD2
• Identification du gène (NCBI): 126259
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane and immunoglobulin domain-containing protein 2 (TMIGD2, also known as CD28H and IGPR1), plays a role in cell-cell interaction, migration, and angiogenesis. Through interaction with HHLA2, it costimulates T-cells in the context of TCR-mediated activation and enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade (PMID: 22419821; 23784006). TMIGD2 has been associated with several diseases, particularly in the context of cancer. It is aberrantly expressed by human AML cells and can be used to identify and enrich functional LSCs (PMID: 38167704). The predicted molecular weight of the TMIGD2 protein is 31 kDa. The higher molecular weight of TMIGD2 could be associated with its potential glycosylation at its extracellular region (PMID: 22419821).

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