Anticorps Polyclonal de lapin anti-TMOD3

TMOD3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12521-1-AP

Synonymes

Tropomodulin 3, Tropomodulin-3, UTMOD, U-Tmod

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Galerie de données de validation

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  WB analysis of Jurkat using 12521-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 12521-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12521-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12521-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12521-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12521-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12521-1-AP (TMOD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12521-1-AP

  IP result of anti-TMOD3 (IP:12521-1-AP, 4ug; Detection:12521-1-AP 1:700) with Jurkat cells lysate 4000 ug.

• 

  IHC staining of human kidney using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12521-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12521-1-AP (TMOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, tissu cardiaque humain, tissu cérébral de souris, tissu pulmonaire humain, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IHC: tissu rénal humain, tissu cardiaque de souris, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

12521-1-AP cible TMOD3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMOD3 Protéine recombinante Ag3215
• Nom complet: tropomodulin 3 (ubiquitous)
• Masse moléculaire calculée: 352 aa, 40 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC020542
• Symbole du gène: TMOD3
• Identification du gène (NCBI): 29766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tropomodulin 3(TMOD3) belongs to the tropomodulin family, which controls cytoskeletal dynamics by regulating the end-capping of actin filaments. TMOD3 bridges the gap between structural integrity and signaling cascades. TMOD3 regulates cytoskeletal architecture and is implicated in cell adhesion, migration, and mechanotransduction.

Publications

Species	Application	Title
human	WB	Carcinogenesis; MiR-145 modulates the radiosensitivity of non-small cell lung cancer cells by suppression of TMOD3.; Authors - Hang Li
human	IHC	Comb Chem High Throughput Screen; Clinicopathological Characteristics, Prognosis, and Correlated Tumor Cell Function of Tropomodulin-3 in Pancreatic Adenocarcinoma; Authors - Bin Zhong
human	WB,IHC	Drug Resist Updat; TMOD3 accelerated resistance to immunotherapy in KRAS-mutated pancreatic cancer through promoting autophagy-dependent degradation of ASCL4; Authors - Zhiwei He

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