Anticorps Monoclonal anti-TMPO/LAP2

TMPO/LAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

3G4E1

N° de cat : 67157-1-Ig

Synonymes

LAP2, TMPO, 3G4E1, Lamina-associated polypeptide 2, isoforms beta/gamma, Thymopentin

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Galerie de données de validation

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  WB analysis using 67157-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67157-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67157-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 67157-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67157-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules K-562, cellules SH-SY5Y
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67157-1-Ig cible TMPO/LAP2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMPO/LAP2 Protéine recombinante Ag6415
• Nom complet: thymopoietin
• Masse moléculaire calculée: 51 kDa
• Poids moléculaire observé: 51 kDa, 39 kDa
• Numéro d’acquisition GenBank: BC053675
• Symbole du gène: TMPO/LAP2
• Identification du gène (NCBI): 7112
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

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