Anticorps Polyclonal de lapin anti-TMPRSS2

TMPRSS2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 14437-1-AP

Synonymes

PP9284, PRSS10, Serine protease 10, TMPRSS2

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Galerie de données de validation

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  WB analysis using 14437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14437-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14437-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14437-1-AP (TMPRSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14437-1-AP (TMPRSS2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14437-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14437-1-AP (TMPRSS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 14437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of human tonsillitis using 14437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMPRSS2 antibody (14437-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules Caco-2, cellules T-47D
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de la prostate humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu d'amygdalite humain,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 7 publications below

Informations sur le produit

14437-1-AP cible TMPRSS2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMPRSS2 Protéine recombinante Ag5824
• Nom complet: transmembrane protease, serine 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 70, 54, 31 kDa
• Numéro d’acquisition GenBank: BC051839
• Symbole du gène: TMPRSS2
• Identification du gène (NCBI): 7113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

Publications

Species	Application	Title
human	WB	Nat Microbiol; SARS-CoV-2 Omicron is an immune escape variant with an altered cell entry pathway.; Authors - Brian J Willett
human	WB	Allergy; Distinct expression of SARS-CoV-2 receptor ACE2 correlates with endotypes of chronic rhinosinusitis with nasal polyps.; Authors - Ming Wang
human	WB	Eur Respir J; Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.; Authors - Kelly S Schweitzer
human	WB	Theranostics; Environmentally-induced mdig contributes to the severity of COVID-19 through fostering expression of SARS-CoV-2 receptor NRPs and glycan metabolism.; Authors - Qian Zhang
human	WB	Mol Ther Nucleic Acids; The accessible promoter-mediated supplementary effect of host factors provides new insight into the tropism of SARS-CoV-2.; Authors - Guifang Du
human	IF	PLoS Pathog; Infection of human Nasal Epithelial Cells with SARS-CoV-2 and a 382-nt deletion isolate lacking ORF8 reveals similar viral kinetics and host transcriptional profiles.; Authors - Akshamal M Gamage

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (12-14-2021)

I transfected HEK293T cells with a plasmid encoding the long isoform of TMPRSS2 with a FLAG tag at the C terminus. I analysed the expression of TMPRSS2 (not expressed endogenously by HEK293T) with the TMPRSS2 antibody but did not detect any difference between transfected and untransfected cells, whereas I could clearly detect it with an anti FLAG antibody. So this antibody might work better on endogenous TMPRSS2 and not very well against my tagged version?

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: transfected HEK293T