Anticorps Monoclonal anti-TMPRSS2

TMPRSS2 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1H7E9

N° de cat : 68264-1-Ig

Synonymes

FLJ41954, PP9284, PRSS10, Serine protease 10, TMPRSS2

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Galerie de données de validation

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  WB analysis using 68264-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68264-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig kidney using 68264-1-Ig

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68264-1-Ig (TMPRSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules Caco-2, cellules COLO 320, cellules HT-29, cellules LNCaP, tissu rénal de porc, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68264-1-Ig cible TMPRSS2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TMPRSS2 Protéine recombinante Ag5824
• Nom complet: transmembrane protease, serine 2
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC051839
• Symbole du gène: TMPRSS2
• Identification du gène (NCBI): 7113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMPRSS2, also named as PRSS10, is a type II transmembrane serine protease which is highly expressed by the epithelium of the human prostate gland. TMPRSS2 may contribute to prostate tumour metastasis via the activation of PAR-2. TMPRSS2 is a Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions. TMPRSS2 is a host cell factor that is critical for the spread of several clinically relevant viruses, including influenza A viruses and coronaviruses (PMID: 23468491, 30626688). SARS-CoV-2 uses the SARS-CoV receptor ACE2 for entry and the serine protease TMPRSS2 for S protein priming. The initial spike protein priming by TMPRSS2 is essential for the entry and viral spread of SARS-CoV-2 through interaction with the ACE2 receptor (PMID: 32142651, 30626688 ). Camostat mesylate, an inhibitor of TMPRSS2, can block SARS-CoV-2 infection of lung cells (PMID: 32142651). The MW of TMPRSS2 is about 65-70 kDa. It can be cleaved into some chains with MW 54 kDa, 31 kDa and 26 kDa (PMID: 25734995, 20382709, 26018085).

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