Anticorps Polyclonal de lapin anti-Thymosin beta 4

• Phare
• Validé par KD/KO

Thymosin beta 4 Polyclonal Antibody for IF, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 19850-1-AP

Synonymes

FX, PTMB4, Seraspenide, T beta 4, TB4X, THYB4, Thymosin beta 4, thymosin beta 4, X linked, Thymosin beta-4, TMSB4, TMSB4X

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Galerie de données de validation

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  IP experiment of mouse skeletal muscle using 19850-1-AP

  IP Result of anti-TMSB4X (IP:19850-1-AP, 4ug; Detection:19850-1-AP 1:300) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (TMSB4X antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (TMSB4X antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 19850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 19850-1-AP (Thymosin beta 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 19850-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Thymosin beta 4 antibody (19850-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 19850-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Thymosin beta 4 antibody (19850-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cardiaque de souris,

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

19850-1-AP cible Thymosin beta 4 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Thymosin beta 4 Protéine recombinante Ag13914
• Nom complet: thymosin beta 4, X-linked
• Masse moléculaire calculée: 63 aa, 7 kDa
• Poids moléculaire observé: 5 kDa
• Numéro d’acquisition GenBank: BC001631
• Symbole du gène: TMSB4X
• Identification du gène (NCBI): 7114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).

Publications

Species	Application	Title
human	IHC	Nat Commun; Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma; Authors - Weilin Pu
chicken	IF	Elife; Single-cell proteomics reveals changes in expression during hair-cell development.; Authors - Ying Zhu
human	WB	J Mol Cell Biol; Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.; Authors - Zhou Yizhuo Y ; KD Validated
mouse	WB	Haematologica; Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.; Authors - Inga Scheller ; KO Validated
human	IHC,IF	Cancer Gene Ther; Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X; Authors - Xiaoyi Zhang
human	IHC	Sci Rep; Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.; Authors - Li-Hsing Chi