Anticorps Polyclonal de lapin anti-TMUB1

TMUB1 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 16638-1-AP

Synonymes

C7orf21, DULP, HOPS, SB144, TMUB1, Ubiquitin like protein DULP, Ubiquitin like protein SB144, UNQ763/PRO1555

---

Galerie de données de validation

• 

  WB analysis using 16638-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16638-1-AP (TMUB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of L02 using 16638-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using TMUB1 antibody (16638-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules L02
• Résultats positifs en IF/ICC: cellules L02,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

16638-1-AP cible TMUB1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMUB1 Protéine recombinante Ag9988
• Nom complet: transmembrane and ubiquitin-like domain containing 1
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 27 kDa, 24 kDa
• Numéro d’acquisition GenBank: BC000936
• Symbole du gène: TMUB1
• Identification du gène (NCBI): 83590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1), also referred to as hepatocyte odd protein shuttling (HOPS), is a transmembrane ubiquitin-like protein that was originally identified in the regenerated liver. HOPS is a transmembrane protein released into the cell by an enzymatic cleavage. This allows HOPS to be shuttled from the nucleus to the cytoplasm and vice versa, depending on cell cycle and environmental stress conditions. HOPS cDNA expression gives rise to the translation of three different proteins with distinct molecular weights of 27, 24 and 21 kDa, respectively.The three isoforms of HOPS are differentially expressed depending on the tissue being examined. In the brain, the shortest isoform prevails over the other, whereas in the intestine the higher molecular weight isoform prevails among the other two. Indeed, HOPS shows a different concentration of the three isoforms, which can play different cell-dependent functions. The ratio between different isoforms may vary owing to physiopathological changes in the cell (PMID:32860479).16638-1-AP can detect two bands and some literature also detected two bands (PMID:30610893, 29777440, 29967478)