Anticorps Polyclonal de lapin anti-TMX1

TMX1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27489-1-PBS

Synonymes

Thioredoxin-related transmembrane protein 1, Thioredoxin domain-containing protein 1, PSEC0085, Protein disulfide-isomerase TMX1, PDIA11

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Galerie de données de validation

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  WB analysis using 27489-1-AP (same clone as 27489-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  WB analysis using 27489-1-AP (same clone as 27489-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27489-1-AP (TMX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27489-1-AP (same clone as 27489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 27489-1-AP (same clone as 27489-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27489-1-AP (TMX1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27489-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27489-1-PBS cible TMX1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMX1 Protéine recombinante Ag26595
• Nom complet: thioredoxin-related transmembrane protein 1
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC036460
• Symbole du gène: TMX1
• Identification du gène (NCBI): 81542
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TMX1, one of the few transmembrane members of the family, is the first vascular thiol isomerase with antithrombotic function. It forms functional complexes with the ER lectin calnexin and preferentially intervenes during maturation of cysteine-containing, membrane-associated proteins while ignoring the same cysteine-containing ectodomains if not anchored at the ER membrane. As such, TMX1 is the first example of a topology-specific client protein redox catalyst in living cells(PMID: 30655304). Covalent modification with AMS increases the molecular mass of originally oxidized species by ~0.5 kDa per thiol group, which allows enhanced separation from the reduced form in electrophoresis(PMID: 29123984). TMX1 has 2 bands produces by redox with the MW of 30-32 kDa.

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