Anticorps Polyclonal de lapin anti-TMX4

TMX4 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 21348-1-AP

Synonymes

KIAA1162, PDIA14, TMX4, TXNDC13, UNQ475/PRO938

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Galerie de données de validation

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  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21348-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21348-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 21348-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 21348-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 21348-1-AP (TMX4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21348-1-AP (TMX4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21348-1-AP

  Immunofluorescent analysis of HepG2 cells using 21348-1-AP (TMX4 antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 21348-1-AP

  1X10^6 HepG2 cells were stained with .2ug TMX4 antibody (21348-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A375, cellules L02, tissu cardiaque humain
• Résultats positifs en IHC: tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

21348-1-AP cible TMX4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TMX4 Protéine recombinante Ag15696
• Nom complet: thioredoxin-related transmembrane protein 4
• Masse moléculaire calculée: 349 aa, 39 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC033787
• Symbole du gène: TMX4
• Identification du gène (NCBI): 56255
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4.

Publications

Species	Application	Title
	WB,IF	Nat Commun; TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress; Authors - Marika K Kucińska
human	WB	J Proteome Res; Mapping of the N-linked glycoproteome of human spermatozoa.; Authors - Wang Gaigai G