Anticorps Polyclonal de lapin anti-TNC/Tenascin-C

TNC/Tenascin-C Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27789-1-PBS

Synonymes

Tenascin, TNC, Glioma-associated-extracellular matrix antigen, GMEM, GP 150 225

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Galerie de données de validation

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  WB analysis using 27789-1-AP (same clone as 27789-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

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  WB analysis using 27789-1-AP (same clone as 27789-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 27789-1-AP (same clone as 27789-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27789-1-AP (TNC/Tenascin-C antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27789-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27789-1-PBS cible TNC/Tenascin-C dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNC/Tenascin-C Protéine recombinante Ag27122
• Nom complet: tenascin C
• Masse moléculaire calculée: 2201 aa, 241 kDa
• Poids moléculaire observé: 220-350 kDa, 190-240 kDa
• Numéro d’acquisition GenBank: BC151843
• Symbole du gène: Tenascin C
• Identification du gène (NCBI): 3371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein of the tenascin family (PMID: 21818551). It is a multimodular protein containing multiple epidermal growth factor (EGF)-like repeats and fibronectin type III (FN III) domains (PMID: 1719530). Tenascin-C is highly expressed during embryonic development, particularly in the developing central nervous system, around motile cells and at epithelial-mesenchymal interaction sites (PMID: 25738825). In adult tissues, the expression and the distribution of TNC are typically limited under normal physiological conditions. It is upregulated during injury, inflammation, regeneration, and cancer (PMID: 7694605; 25120494). Tenascin-C is a diverse protein that can produce different functions including regulating cell adhesion, migration and proliferation.

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