Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240702A5

N° de cat : 80260-2-PBS

Synonymes

Tnf, Tnfa, DIF, TNF a, TNF alpha

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Galerie de données de validation

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  IHC staining of mouse spleen using 80260-2-RR (same clone as 80260-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80260-2-RR (Tnfa antibody) at dilution of 1:2000 (under 10x lens). This data was developed using the same antibody clone with 80260-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse spleen using 80260-2-RR (same clone as 80260-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 80260-2-RR (Tnfa antibody) at dilution of 1:2000 (under 40x lens). This data was developed using the same antibody clone with 80260-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80260-2-PBS cible TNF-alpha dans les applications de IHC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNF-alpha Protéine recombinante Eg31366
• Nom complet: tumor necrosis factor
• Numéro d’acquisition GenBank: NM-013693
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 21926
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.

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