Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

5H8

N° de cat : 82863-3-PBS

Synonymes

Tnf, Cachectin, TNF alpha, TNF alpha,TNF-a,TNF-alpha,TNFα, TNF α

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP02049-1

  Sandwich ELISA standard curve of MP02049-1, Rat TNF-alpha Recombinant Matched Antibody Pair - PBS only. 82863-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31374. 82863-7-PBS was HRP conjugated as the detection antibody. Range: 7.81-500 pg/mL.

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  Sample test of MP02049-1

  Rat spleens were cut into 1-2 mm pieces and cultured unstimulated or stimulated with 5 μg/mL ConA for 2 days. The mean TNF-alpha concentration was not determined in unstimulated supernatant, 118.8 pg/mL in ConA stimulated supernatant.

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  Affinity and Kinetic Characterization of 82863-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 82863-3-PBSagainst Rat TNF-alpha was performed. The affinity constant is below 1 pM.

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Informations sur le produit

82863-3-PBS cible TNF-alpha dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNF-alpha Protéine recombinante Eg31374
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Numéro d’acquisition GenBank: BC107671
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 24835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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