Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240668A4

N° de cat : 83721-6-PBS

Synonymes

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00702-4

  Sandwich ELISA standard curve of MP00702-4, TNF-alpha Recombinant Matched Antibody Pair - PBS only. 83721-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0816. 83721-6-PBS was HRP conjugated as the detection antibody. Range: 3.9-250 pg/mL.

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  Sample test of MP00702-4

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean TNF-alpha concentration was determined to be 1.3 pg/mL in unstimulated PBMC supernatant,1236.3 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00702-4

  Serum of seven individual healthy human donors was measured. The human TNF-alpha concentration of detected samples was determined to be 3.25 pg/mL with a range of ND-9.1 pg/mL.

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  Affinity and Kinetic Characterization of 83721-6-PBS

  Biolayer interferometry (BLl) kinetic assay of 83721-6-PBS against Human TNF-alpha was performed. The affinity constant is 47.9 pM.

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Informations sur le produit

83721-6-PBS cible TNF-alpha dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Masse moléculaire calculée: 26 kDa
• Numéro d’acquisition GenBank: NM_000594.4
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 7124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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