Anticorps Recombinant de lapin anti-TNF-alpha

TNF-alpha Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240668B4

N° de cat : 83721-7-RR

Synonymes

TNF, TNF alpha, TNF-a, 240668B4, C-domain 1

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Galerie de données de validation

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  WB analysis of THP-1 using 83721-7-RR

  Untreated, LPS and Brefeldin A treated THP-1 cells were subjected to SDS PAGE followed by western blot with 83721-7-RR (TNF-alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83721-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83721-7-RR against Human TNF-alpha were performed. The affinity constant is 8.51 nM.

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules THP-1 traitées au LPS

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83721-7-RR cible TNF-alpha dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: NM_000594.4
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 7124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

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