Anticorps Monoclonal anti-TNF Alpha

TNF Alpha Monoclonal Antibody for IF

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IF

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7B8A11

N° de cat : CL488-60291

Synonymes

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



Applications testées

Résultats positifs en IFtissu de cancer du sein humain,

Dilution recommandée

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-60291 cible TNF Alpha dans les applications de WB, IF et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéesouris
Hôte / Isotype Mouse / IgG2b
Clonalité Monoclonal
Type Anticorps
Immunogène TNF Alpha Protéine recombinante Ag11413
Nom complet tumor necrosis factor (TNF superfamily, member 2)
Masse moléculaire calculée 233 aa, 26 kDa
Numéro d’acquisition GenBankBC028148
Symbole du gène TNF
Identification du gène (NCBI) 7124
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocole

Product Specific Protocols
IF protocol for CL Plus 488 TNF Alpha antibody CL488-60291Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Biomed Pharmacother

Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage.

Authors - Qing Xiong
mouseIF

Biomacromolecules

Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis

Authors - Qiumeng Chen