Anticorps Monoclonal anti-TNF-alpha

• Phare
• Validé par KD/KO

TNF-alpha Monoclonal Antibody for IF-P

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF-P

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

Publications(6)

CloneNo.

7B8A11

N° de cat : CL488-60291

Synonymes

TNF, TNF alpha, TNF-a, Intracellular domain 2, Intracellular domain 1

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Galerie de données de validation

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  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

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  IF Staining of human breast cancer using CL488-60291

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CL488-60291 (TNF alpha antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en IF-P: tissu de cancer du sein humain,

Dilution recommandée

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

CL488-60291 cible TNF-alpha dans les applications de WB, IHC, IF-P et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNF-alpha Protéine recombinante Ag11413
• Nom complet: tumor necrosis factor (TNF superfamily, member 2)
• Masse moléculaire calculée: 233 aa, 26 kDa
• Numéro d’acquisition GenBank: BC028148
• Symbole du gène: TNF-alpha
• Identification du gène (NCBI): 7124
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Publications

Species	Application	Title
human	IF	PLoS Pathog; SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure; Authors - Huilong Li
	WB	Biomed Pharmacother; Nr2e1 ablation impairs liver glucolipid metabolism and induces inflammation, high-fat diets amplify the damage.; Authors - Qing Xiong
mouse	IF	Foods; Effects of Dietary Pretreatment with All-trans Lycopene on Lipopolysaccharide-Induced Jejunal Inflammation: A Multi-Pathway Phenomenon; Authors - Daolin Mou
	IF	Neural Regen Res; Changes in border-associated macrophages after stroke: single-cell sequencing analysis; Authors - Ning Yu
mouse	IF	Biomacromolecules; Multifunctional Polymer Vesicles for Synergistic Antibiotic-Antioxidant Treatment of Bacterial Keratitis; Authors - Qiumeng Chen
mouse	IHC,IF	Front Pharmacol; Licoricesaponin G2 ameliorates bleomycin-induced pulmonary fibrosis via targeting TNF-α signaling pathway and inhibiting the epithelial-mesenchymal transition; Authors - Jing Ma

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