Anticorps Polyclonal de lapin anti-TNF Alpha
TNF Alpha Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (4)
Applications
WB, Transwell Assay, Cell treatment, ELISA
Conjugaison
Non conjugué
533
N° de cat : 17590-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules THP-1, cellules RAW 264.7 traitées au LPS |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 436 publications below |
ELISA | See 3 publications below |
Informations sur le produit
17590-1-AP cible TNF Alpha dans les applications de WB, Transwell Assay, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain, souris
Réactivité | Humain, souris |
Réactivité citée | rat, bovin, Humain, Lapin, souris, fish |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | TNF Alpha Protéine recombinante Ag11433 |
Nom complet | tumor necrosis factor (TNF superfamily, member 2) |
Masse moléculaire calculée | 233 aa, 26 kDa |
Poids moléculaire observé | 26 kDa |
Numéro d’acquisition GenBank | BC028148 |
Symbole du gène | TNF |
Identification du gène (NCBI) | 7124 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.
Protocole
Product Specific Protocols | |
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WB protocol for TNF Alpha antibody 17590-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Behav Immun HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway. | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Small M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation | ||
Mater Today Bio Dexamethasone-loaded zeolitic imidazolate frameworks nanocomposite hydrogel with antibacterial and anti-inflammatory effects for periodontitis treatment | ||
Acta Biomater Mild hyperthermia promotes immune checkpoint blockade-based immunotherapy against metastatic pancreatic cancer using size-adjustable nanoparticles. | ||
Redox Biol Icariin alleviates uveitis by targeting peroxiredoxin 3 to modulate retinal microglia M1/M2 phenotypic polarization. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Margarita (Verified Customer) (11-22-2023) | Prostate Cancer cell line, overnight incubation, Mouse
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FH Isha (Verified Customer) (09-20-2021) | signal was very good. My sample was renal tissue lysate after ischemia reperfusion
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