Anticorps Monoclonal anti-TNFAIP3

TNFAIP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

3A11G6

N° de cat : 66695-1-Ig

Synonymes

A20, A20p37, A20p50, EC:2.3.2.-, OTU domain-containing protein 7C

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Galerie de données de validation

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  WB analysis using 66695-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66695-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat ovary using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat ovary using 66695-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66695-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TNFAIP3 antibody (66695-1-Ig, Clone: 3A11G6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

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  FC experiment of HeLa using 66695-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TNFAIP3 (66695-1-Ig, Clone:3A11G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat
• Résultats positifs en IHC: tissu rénal de rat, tissu de cancer de la thyroïde humain, tissu ovarien de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

66695-1-Ig cible TNFAIP3 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNFAIP3 Protéine recombinante Ag18150
• Nom complet: tumor necrosis factor, alpha-induced protein 3
• Masse moléculaire calculée: 790 aa, 90 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC114480
• Symbole du gène: TNFAIP3
• Identification du gène (NCBI): 7128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Basis Dis; Rhythm gene PER1 mediates ferroptosis and lipid metabolism through SREBF2/ALOX15 axis in polycystic ovary syndrome; Authors - Yuanyuan Chen
mouse	IHC	Biology (Basel); Interleukin-10 Deficiency Impacts on TNF-Induced NFκB Regulated Responses In Vivo; Authors - Stamatia Papoutsopoulou
human	WB,IHC,IF	Cell Death Dis; A20 targets PFKL and glycolysis to inhibit the progression of hepatocellular carcinoma.; Authors - Yilu Feng
human,mouse	IHC,IF	Sci Total Environ; Alpha-ketoglutarate alleviates cadmium-induced inflammation by inhibiting the HIF1A-TNFAIP3 pathway in hepatocytes; Authors - Yinzhao Jia
	IF	Immunity; Integrated computational analysis identifies therapeutic targets with dual action in cancer cells and T cells; Authors - Ce Luo
		Invest Ophthalmol Vis Sci; Global MicroRNA Profiling of HSV-1 Infected Cornea Identifies miR-329 as a Novel Regulator of Virus Infection; Authors - Pankaj Sharma

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