Anticorps Monoclonal anti-TNFAIP3

TNFAIP3 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3A11G6

N° de cat : 66695-1-PBS

Synonymes

A20, A20p37, A20p50, EC:2.3.2.-, OTU domain-containing protein 7C

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Galerie de données de validation

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  WB analysis using 66695-1-Ig (same clone as 66695-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66695-1-Ig (same clone as 66695-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IHC staining of rat ovary using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 66695-1-Ig (TNFAIP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66695-1-Ig (same clone as 66695-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TNFAIP3 antibody (66695-1-Ig, Clone: 3A11G6 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66695-1-Ig (same clone as 66695-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human TNFAIP3 (66695-1-Ig, Clone:3A11G6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66695-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66695-1-PBS cible TNFAIP3 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNFAIP3 Protéine recombinante Ag18150
• Nom complet: tumor necrosis factor, alpha-induced protein 3
• Masse moléculaire calculée: 790 aa, 90 kDa
• Poids moléculaire observé: 80 kDa
• Numéro d’acquisition GenBank: BC114480
• Symbole du gène: TNFAIP3
• Identification du gène (NCBI): 7128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TNFAIP3, also named A20, is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B (NFKB) activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 is a ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities, and involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity.

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