Anticorps Polyclonal de lapin anti-TSG-6

• Phare
• Validé par KD/KO

TSG-6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 13321-1-AP

Synonymes

TNFAIP6, EC:3.1.1.-, Hyaluronate binding protein, Hyaluronate-binding protein, TNF alpha induced protein 6

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 13321-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3,
• Résultats positifs en IHC: tissu rénal de rat, tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

13321-1-AP cible TSG-6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSG-6 Protéine recombinante Ag3885
• Nom complet: tumor necrosis factor, alpha-induced protein 6
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC030205
• Symbole du gène: TNFAIP6
• Identification du gène (NCBI): 7130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSG-6 was first discovered by Lee when screening the cDNA expression library of human fibroblasts interfered by TNF-α. TSG-6 is expressed in normal fibroblasts, peripheral blood mononuclear cells, synovial cells and chondrocytes. It is constitutively expressed in tissue cells with immune barrier, high basal metabolism level and differentiation potential (such as mesenchymal stem cells), but it is usually not expressed in normal tissue cells, but can be induced by inflammatory mediators. TSG-6 has the functions of anti-inflammation, extracellular matrix remodeling and local microenvironment protection. It plays an important role in the repair of joint inflammation by binding with various ligands, including glycosaminoglycan, proteoglycan core protein and other matrix components, and directly binding with various chemokines and bone morphogenetic proteins. TSG-6 has shown anti-inflammatory effects in many diseases, including skin wound closure, pancreatitis, conjunctivitis, neuritis, intestinal ischemia-reperfusion injury, liver fibrosis, acute myocardial infarction and so on. It is the secreted protein product of tumor necrosis factor-stimulating gene-6 (TSG-6) with a molecular mass of about 35-38 kDa.(PMID: 34707499)

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci; Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.; Authors - Rena Watanabe
mouse	WB	Front Pharmacol; TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.; Authors - Yue-Meng Wan
rat	WB	Neurochem Res; Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.; Authors - Bin Tang
human	WB,IHC	Contrast Media Mol Imaging; TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway; Authors - Hongquan Cui ; KD Validated
human	WB,IHC,IF	Neural Regen Res; Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis; Authors - Mingxiang Ding
human	WB,IHC	Front Immunol; Neutrophil infiltration associated genes on the prognosis and tumor immune microenvironment of lung adenocarcinoma; Authors - Renwang Liu

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