Anticorps Polyclonal de lapin anti-TSG-6

• Phare
• Validé par KD/KO

TSG-6 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13321-1-AP

Synonymes

Hyaluronate binding protein, TNF alpha induced protein 6, TNF stimulated gene 6 protein, TNFAIP6, TSG 6, TSG6, TSG-6

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 13321-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13321-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13321-1-AP (TSG-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 13321-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13321-1-AP (TSG-6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules A549
• Résultats positifs en IHC: tissu rénal de rat, tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

13321-1-AP cible TSG-6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TSG-6 Protéine recombinante Ag3885
• Nom complet: tumor necrosis factor, alpha-induced protein 6
• Masse moléculaire calculée: 277 aa, 31 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC030205
• Symbole du gène: TNFAIP6
• Identification du gène (NCBI): 7130
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	JACC Basic Transl Sci; Atheroprotective Effects of Tumor Necrosis Factor-Stimulated Gene-6.; Authors - Rena Watanabe
mouse	WB	Front Pharmacol; TSG-6 Inhibits Oxidative Stress and Induces M2 Polarization of Hepatic Macrophages in Mice With Alcoholic Hepatitis via Suppression of STAT3 Activation.; Authors - Yue-Meng Wan
rat	WB	Neurochem Res; Tumor Necrosis Factor-stimulated Gene-6 (TSG-6) Secreted by BMSCs Regulates Activated Astrocytes by Inhibiting NF-κB Signaling Pathway to Ameliorate Blood Brain Barrier Damage After Intracerebral Hemorrhage.; Authors - Bin Tang
human	WB,IHC	Contrast Media Mol Imaging; TNFAIP6 Promotes Gastric Carcinoma Cell Invasion via Upregulating PTX3 and Activating the Wnt/β-Catenin Signaling Pathway; Authors - Hongquan Cui ; KD Validated
human	WB,IHC,IF	Neural Regen Res; Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis; Authors - Mingxiang Ding