Anticorps Polyclonal de lapin anti-TNFR1

• Phare
• Validé par KD/KO

TNFR1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

48

Avis (1)

N° de cat : 21574-1-AP

Synonymes

CD120a, FPF, p55, p55 R, p60, TBP1, TBPI, TNF R, TNF R I, TNF R1, TNF R55, TNF Receptor 1, TNF Receptor I, TNF RI, TNFAR, TNFR I, TNFR1, TNFR55, TNFR60, TNFRSF1A

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Galerie de données de validation

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  WB analysis of Raji using 21574-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21574-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21574-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21574-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21574-1-AP (TNFR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21574-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21574-1-AP (TNFR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 21574-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TNFR1 antibody (21574-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21574-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TNFR1 antibody (21574-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Raji using 21574-1-AP

  1X10^6 Raji cells were stained with 0.2ug TNFR1 antibody (21574-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules HL-60, tissu cérébral humain
• Résultats positifs en IHC: tissu cérébral humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 40 publications below
• IHC: See 7 publications below
• IF: See 13 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

21574-1-AP cible TNFR1 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNFR1 Protéine recombinante Ag16112
• Nom complet: tumor necrosis factor receptor superfamily, member 1A
• Masse moléculaire calculée: 455 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC010140
• Symbole du gène: TNFRSF1A
• Identification du gène (NCBI): 7132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

Publications

Species	Application	Title
human	WB	Aging Dis; MiR-29a-3p Improves Acute Lung Injury by Reducing Alveolar Epithelial Cell PANoptosis.; Authors - Yanhui Cui
mouse	WB	PLoS Biol; A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.; Authors - Yingying Zhang
human	WB	Cell Death Discov; Repositioning linifanib as a potent anti-necroptosis agent for sepsis; Authors - Liang Yu
human	WB,IP	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
mouse	WB,IF,IP	Free Radic Biol Med; Kv7.4 channel is a key regulator of vascular inflammation and remodeling in neointimal hyperplasia and abdominal aortic aneurysms.; Authors - Xi-Zhenzi Fan
human	IF	Neural Regen Res; Transplantation of fibrin-thrombin encapsulated human induced neural stem cells promotes functional recovery of spinal cord injury rats through modulation of the microenvironment; Authors - Sumei Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tinne (Verified Customer) (08-14-2023)

This AB worked quite well - not the strongest signal - might be worth increasing the concentration. I got the best results when I did not permeabilise the cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human hippocampal progenitor cells

TNFR1 Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:21574-1-AP)

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