Anticorps Monoclonal anti-TNFR1/CD120a

• Phare
• Validé par KD/KO

TNFR1/CD120a Monoclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

2A6E3

N° de cat : 60192-1-PBS

Synonymes

CD120a, TNFR1, TNFRSF1A, 2A6E3, FPF

---

Galerie de données de validation

• 

  WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1/CD120a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60192-1-Ig (same clone as 60192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 60192-1-Ig (same clone as 60192-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 60192-1-Ig (same clone as 60192-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 60192-1-Ig (TNFR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1/CD120a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60192-1-Ig (TNFR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 60192-1-Ig (same clone as 60192-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TNFR1/CD120a antibody (60192-1-Ig, Clone: 2A6E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60192-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

60192-1-PBS cible TNFR1/CD120a dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: TNFR1/CD120a Protéine recombinante Ag16112
• Nom complet: tumor necrosis factor receptor superfamily, member 1A
• Masse moléculaire calculée: 455 aa, 50 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC010140
• Symbole du gène: TNFR1
• Identification du gène (NCBI): 7132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

{{ptg:RelatedPrimaryAntibodies}}