Anticorps Recombinant de lapin anti-TNFR1/CD120a

TNFR1/CD120a Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241336B4

N° de cat : 84243-5-RR

Synonymes

CD120a, TNFR1, TNFRSF1A, 241336B4, FPF

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Galerie de données de validation

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  WB analysis using 84243-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84243-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 84243-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 84243-5-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84243-5-RR (TNFR1/CD120a antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of Jurkat using 84243-5-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using TNFR1/CD120a antibody (84243-5-RR, Clone: 241336B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84243-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84243-5-RR against Human TNFR1/CD120a were performed. The affinity constant is 20.5 nM.

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Applications testées

• Résultats positifs en WB: Calu-1 cells, cellules HL-60, cellules NIH/3T3, cellules U2OS
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84243-5-RR cible TNFR1/CD120a dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: tumor necrosis factor receptor superfamily, member 1A
• Masse moléculaire calculée: 455 aa, 50 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC010140
• Symbole du gène: TNFR1
• Identification du gène (NCBI): 7132
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNF exists in soluble and membrane-bound forms. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). Whereas TNFR1 is widely expressed, expression of TNFR2 is limited to cells of the immune system, endothelial cells, and nerve cells (PMID: 22053109). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling (PMID: 16407280). This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

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