Anticorps Polyclonal de lapin anti-TNFR2 / TNFRSF1B

• Phare
• Validé par KD/KO

TNFR2 / TNFRSF1B Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

28

Avis (1)

N° de cat : 19272-1-AP

Synonymes

CD120b, Etanercept, p75, p75TNFR, p80 TNF alpha receptor, TBP 2, TBPII, TNF R II, TNF R2, TNF R75, TNF Receptor 2, TNF Receptor II, TNF RII, TNFBR, TNFR II, TNFR1B, TNFR2, TNFR80, TNFRSF1B

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Galerie de données de validation

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  WB analysis using 19272-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19272-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19272-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19272-1-AP (TNFR2 / TNFRSF1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19272-1-AP

  IP Result of anti-TNFR2 / TNFRSF1B (IP:19272-1-AP, 4ug; Detection:19272-1-AP 1:500) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 19272-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19272-1-AP (TNFR2 / TNFRSF1B Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 19272-1-AP

  Immunofluorescent analysis of Hela cells, using TNFR2 / TNFRSF1B antibody 19272-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 19272-1-AP

  1X10^6 HeLa cells were stained with 0.5ug TNFR2 / TNFRSF1B antibody (19272-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Rabbit IgG with dilution 1:100.

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Applications testées

• Résultats positifs en WB: NK-92 cells, cellules HEK-293, cellules Jurkat, cellules THP-1, tissu de thymus de rat, tissu de thymus de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu splénique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.05 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 19 publications below
• IHC: See 8 publications below
• IF: See 11 publications below
• FC: See 1 publications below

Informations sur le produit

19272-1-AP cible TNFR2 / TNFRSF1B dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNFR2 / TNFRSF1B Protéine recombinante Ag5866
• Nom complet: tumor necrosis factor receptor superfamily, member 1B
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC052977
• Symbole du gène: TNFRSF1B
• Identification du gène (NCBI): 7133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

Publications

Species	Application	Title
human	FC,IF	Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Mol Ther; Tumor necrosis factor alpha delivers exogenous inflammation-related microRNAs to recipient cells with functional targeting capabilities.; Authors - Yuechao Zhao
rat	WB	Mater Today Bio; Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis; Authors - Jingzhe Wang
rat	WB	Oxid Med Cell Longev; Protective Effects of Cinnamaldehyde against Mesenteric Ischemia-Reperfusion-Induced Lung and Liver Injuries in Rats.; Authors - Marwan Almoiliqy
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang
mouse	IF	Pain; Nox2-dependent signaling between macrophages and sensory neurons contributes to neuropathic pain hypersensitivity.; Authors - Wiebke Kallenborn-Gerhardt

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tinne (Verified Customer) (08-14-2023)

This worked really well for ICC of human HPCs.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Human hippocampal progenitor cells

TNFR2 / TNFRSF1B Antibody Immunofluorescence validation (1:400 dilution) in Human hippocampal progenitor cells (Cat no:19272-1-AP)

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