Anticorps Recombinant de lapin anti-TNFR2/CD120b

TNFR2/CD120b Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230328B6

N° de cat : 83101-1-PBS

Synonymes

TNFRSF1B, 230328B6, CD120b, Etanercept, p75

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Galerie de données de validation

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  WB analysis using 83101-1-RR (same clone as 83101-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83101-1-RR (TNFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83101-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83101-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83101-1-RR against Human TNFR2 were performed. The affinity constant is 0.43 nM.

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Informations sur le produit

83101-1-PBS cible TNFR2/CD120b dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNFR2/CD120b Protéine recombinante Eg0086
• Nom complet: tumor necrosis factor receptor superfamily, member 1B
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 75 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC052977
• Symbole du gène: TNFR2
• Identification du gène (NCBI): 7133
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

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