Anticorps Polyclonal de lapin anti-Osteoprotegerin/TNFRSF11B

Osteoprotegerin/TNFRSF11B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 11534-1-AP

Synonymes

Tumor necrosis factor receptor superfamily member 11B, TNFRSF11B, Osteoprotegerin, Osteoclastogenesis inhibitory factor, OPG

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Galerie de données de validation

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human milk using 11534-1-AP

  human milk was subjected to SDS PAGE followed by western blot with 11534-1-AP (Osteoprotegerin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 11534-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11534-1-AP (Osteoprotegerin/TNFRSF11B antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11534-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Osteoprotegerin antibody (11534-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: lait humain,
• Résultats positifs en IHC: tissu rénal de rat, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

11534-1-AP cible Osteoprotegerin/TNFRSF11B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Osteoprotegerin/TNFRSF11B Protéine recombinante Ag2139
• Nom complet: tumor necrosis factor receptor superfamily, member 11b
• Masse moléculaire calculée: 401 aa, 46 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC030155
• Symbole du gène: TNFRSF11B
• Identification du gène (NCBI): 4982
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Osteoprotegerin (OPG), also known as tumor necrosis factor receptor superfamily member 11B (TNFRSF11B) and osteoclastogenesis inhibitory factor (OCIF), belongs to the tumor necrosis factor (TNF) receptor superfamily. By binding to RANKL, OPG inhibits osteoclastic activity and promoting bone formationis. OPG plays a key role on cell survival by inhibiting TRAIL-induced apoptosis. OPG also is involved in vascular biology, fibrosis, immunity, EMT, and the apoptosis of cancer cells (PMID: 35523775).

Publications

Species	Application	Title
human	WB	Anal Cell Pathol (Amst); Cartilage Damage Pathological Characteristics of Diabetic Neuropathic Osteoarthropathy; Authors - Pei-Long Liu
rat,human	IHC	BMC Oral Health; Artesunate-loaded thermosensitive chitosan hydrogel promotes osteogenesis of maxillary tooth extraction through regulating T lymphocytes in type 2 diabetic rats; Authors - Jinghong Luo
	WB	Foods; Optimization of the Preparation Process and Ameliorative Efficacy in Osteoporotic Rats of Peptide-Calcium Chelates from Skipjack Tuna (Katsuwonus pelamis) Meat; Authors - Wan-Zhen Yan
rat	WB	J Ethnopharmacol; Regulation of monocyte polarization through Nuclear Factor Kappa B /Inhibitor of Kappa B Alpha pathway by Cuscuta chinensis Lam. in postmenopausal osteoporosis; Authors - Xiaochen Li

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