Anticorps Polyclonal de lapin anti-BCMA/TNFRSF17

BCMA/TNFRSF17 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 27724-1-AP

Synonymes

TNFRSF17, CD269, BCMA, BCM, B-cell maturation protein

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Galerie de données de validation

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  WB analysis of Daudi using 27724-1-AP

  Daudi and Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 27724-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 27724-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 27724-1-AP (TNFRSF17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27724-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27724-1-AP (TNFRSF17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Raji, cellules Ramos
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

27724-1-AP cible BCMA/TNFRSF17 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BCMA/TNFRSF17 Protéine recombinante Ag27049
• Nom complet: tumor necrosis factor receptor superfamily, member 17
• Masse moléculaire calculée: 184 aa, 20 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC058291
• Symbole du gène: TNFRSF17
• Identification du gène (NCBI): 608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BCMA (B cell maturation antigen), also known as TNFRSF17, is 20.2-kDa type III transmembrane glycoprotein and is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes and plasma cells, which may be important for B cell development and autoimmune response (PMID: 32943087; 9846698). BCMA has two agonist ligands: a proliferation-inducing ligand (APRIL) and BAFF. When BCMA binds to APRIL, it transmits signals of cell survival and proliferation (PMID: 27127303); when BCMA binds to BAFF, it mediates the activation of NF-kappaB and MAPK8/JNK (PMID: 36140254). It has been found that the overexpression and activation of BCMA are associated with multiple myeloma (MM) in preclinical models and humans, supporting its potential utility as a therapeutic target for MM (PMID: 32055000; 32943087).

Publications

Species	Application	Title
human	WB,IHC	Eur J Nucl Med Mol Imaging; Discovery of a highly specific radiolabeled antibody targeting B-cell maturation antigen: Applications in PET imaging of multiple myeloma; Authors - Jie Ma
human,mouse	WB,IHC	BMC Cancer; 25-hydroxycholesterol promotes proliferation and metastasis of lung adenocarcinoma cells by regulating ERβ/TNFRSF17 axis; Authors - Mengting He ; KD Validated
human	WB	J Exp Med; Developing high-affinity decoy receptors to treat multiple myeloma and diffuse large B cell lymphoma.; Authors - Yu Rebecca Miao
human	WB	J Med Chem; Development of a Novel Peptide-Based PET Tracer [68Ga]Ga-DOTA-BP1 for BCMA Detection in Multiple Myeloma; Authors - Lele Song

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