Anticorps Monoclonal anti-DR6

DR6 Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

3C2D10

N° de cat : 66754-1-Ig

Synonymes

BM 018, CD358, Death receptor 6, DR6, TNFRSF21

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Galerie de données de validation

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  WB analysis of K-562 using 66754-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66754-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 66754-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 66754-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66754-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66754-1-Ig (DR6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 66754-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DR6 antibody (66754-1-Ig, Clone: 3C2D10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules Daudi, cellules Jurkat, cellules Raji, cellules THP-1
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66754-1-Ig cible DR6 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DR6 Protéine recombinante Ag14327
• Nom complet: tumor necrosis factor receptor superfamily, member 21
• Masse moléculaire calculée: 655 aa, 72 kDa
• Poids moléculaire observé: 69 kDa
• Numéro d’acquisition GenBank: BC021572
• Symbole du gène: TNFRSF21
• Identification du gène (NCBI): 27242
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DR6 (also known as TNFRSF21 or CD358) is an orphan receptor of the tumor necrosis factor (TNF) receptor superfamily. It controls cell death and differentiation in a cell-autonomous manner in different cell types including neurons, oligodendrocytes, endothelial cells, and immune cells (PMID: 29459438). DR6 has been shown to activate NF-κB and JNK, and induce cell apoptosis (PMID: 9714541). DR6 interacts with TRADD, which is known to mediate signal transduction of TNF receptors. It has been reported that APP binds DR6 to trigger axon pruning and neuron death via distinct caspases, indicating that DR6 might be involved in Alzheimer's disease (PMID: 19225519).