Anticorps Polyclonal de lapin anti-CD134/OX40

CD134/OX40 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20006-1-AP

Synonymes

ACT35, ACT35 antigen, CD134, CD134/OX40, OX40, OX40L receptor, TNFRSF4, TXGP1L

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Galerie de données de validation

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  WB analysis of HepG2 using 20006-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20006-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 20006-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20006-1-AP (CD134/OX40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  FC experiment of human PBMCs using 20006-1-AP

  1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or unstained (blue). Unstimulated human PBMCs were surface stained with 0.2ug Anti-Human CD134/OX40 (20006-1-AP) and CoraLite®750-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black dashed). Cells were not fixed. Lymphocytes were gated.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cellules U-937
• Résultats positifs en cytométrie: PHA treated human PBMCs,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

20006-1-AP cible CD134/OX40 dans les applications de WB, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor necrosis factor receptor superfamily, member 4
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: NM_003327
• Symbole du gène: TNFRSF4
• Identification du gène (NCBI): 7293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD134 (OX40) is a member of the TNFR-superfamily of receptors. Predominantly expressed on activated T cells, CD134 is activated by its cognate ligand CD134L (OX40L) and functions as a T cell co-stimulatory molecule. CD134-CD134L interactions have been proposed as a potential therapeutic target for treating autoimmunity (PMID: 26215166). CD134 can interact with TRAF2, TRAF3, and TRAF5.

Publications

Species	Application	Title
human	WB	Front Immunol; A Novel Immune-Related Gene Signature to Identify the Tumor Microenvironment and Prognose Disease Among Patients With Oral Squamous Cell Carcinoma Patients Using ssGSEA: A Bioinformatics and Biological Validation Study; Authors - Yun Chen
human	WB	Dis Markers; Development of a Costimulatory Molecule Signature to Predict Prognosis, Immune Landscape, and Response to Immune Therapy for Hepatocellular Carcinoma; Authors - Yongjie Zhou

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