Anticorps Recombinant de lapin anti-TNFSF11

TNFSF11 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

241791B7

N° de cat : 84431-5-RR

Synonymes

241791B7, CD254, ODF, OPGL, Osteoclast differentiation factor

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Galerie de données de validation

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  WB analysis using 84431-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84431-5-RR (TNFSF11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 84431-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84431-5-RR (TNFSF11 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84431-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84431-5-RR against Mouse TNFSF11 were performed. The affinity constant is 9.78 nM.

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, tissu hépatique de souris
• Résultats positifs en IHC: tissu splénique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84431-5-RR cible TNFSF11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: TNFSF11 Protéine recombinante Eg1956
• Nom complet: tumor necrosis factor (ligand) superfamily, member 11
• Masse moléculaire calculée: 35KD
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_011613.3
• Symbole du gène: Tnfsf11
• Identification du gène (NCBI): 21943
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNFSF11, known as Tumor Necrosis Factor Superfamily Member 11, also known as RANKL (Receptor Activator of Nuclear Factor-κB Ligand), TRANCE, etc., is a member of the Tumor Necrosis Factor (TNF) superfamily.RANKL is a type II homotrimeric transmembrane protein located primarily at the plasma membrane. RANKL is a type II homotrimeric transmembrane protein located mainly at the plasma membrane, and in addition to the membrane-bound form (mRNAKL), it can also be cleaved by proteolytic cleavage of the cell membrane to produce the soluble form (sRANKL). The membrane-bound form of RANKL is the main form involved in triggering the RANKL/RANK signaling pathway and mediating osteoclastogenesis. Current studies have shown that TNFSF11 acts primarily in the immune and skeletal systems and has been implicated in the pathogenesis of degenerative bone diseases, carcinogenesis, and asthma.

Publications

Species	Application	Title
		Exp Cell Res; Activated NETosis of bone marrow neutrophils up-regulates macrophage osteoclastogenesis via cGAS-STING/AKT2 pathway to promote osteoporosis; Authors - Yutong Guo

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