Anticorps Polyclonal de lapin anti-TNIK

TNIK Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 30906-1-AP

Synonymes

EC:2.7.11.1, KIAA0551, TRAF2 and NCK-interacting protein kinase

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Galerie de données de validation

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  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30906-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30906-1-AP (TNIK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 30906-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using TNIK antibody (30906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: COLO 205 cells, cellules HT-29, cellules K-562
• Résultats positifs en IF/ICC: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30906-1-AP cible TNIK dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNIK Protéine recombinante Ag34132
• Nom complet: TRAF2 and NCK interacting kinase
• Poids moléculaire observé: 155~180 kDa
• Numéro d’acquisition GenBank: BC150256
• Symbole du gène: TNIK
• Identification du gène (NCBI): 23043
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNIK (Traf2- and Nck-interacting kinase) is one of the germinal center kinase family members involved in cytoskeleton organization and neuronal dendrite extension, which had been implicated in postsynaptic signaling as well as in regulation of cell proliferation. TNiK is expressed in the nervous system and plays key roles at the synapse and nucleus, In non-neuronal cells, TNiK has been described as a critical component of transcriptional regulatory mechanisms, mediated by Wnt signaling (PMID: 24566388). TNIK is also an essential regulatory component of the T-cell factor-4 and β-catenin transcriptional complex and is required for the tumor-initiating function of colorectal cancer stem cells (PMID: 10521462). Western blot analysis detected TNIK at an apparent molecular mass of 150~180 kDa as cited in literature (PMID: 24566388).

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