Anticorps Polyclonal de lapin anti-TNK1

TNK1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 14199-1-AP

Synonymes

CD38 negative kinase 1, TNK1

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Galerie de données de validation

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  WB analysis using 14199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14199-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14199-1-AP (TNK1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14199-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14199-1-AP (TNK1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HL-60, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14199-1-AP cible TNK1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TNK1 Protéine recombinante Ag5402
• Nom complet: tyrosine kinase, non-receptor, 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC035782
• Symbole du gène: TNK1
• Identification du gène (NCBI): 8711
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; Thirty-Eight-Negative Kinase 1 Is a Mediator of Acute Kidney Injury in Experimental and Clinical Traumatic Hemorrhagic Shock.; Authors - Rebecca Halbgebauer
human,rat	WB, IHC	3 Biotech; Tyrosine kinase nonreceptor 1 (TNK1) knockdown ameliorates hemorrhage shock-induced kidney injury via inhibiting macrophage M1 polarization.; Authors - Miaolong Tang
human	IF	J Gastroenterol; Inhibition of liver cancer cell growth by metabolites S-adenosylmethionine and nicotinic acid originating from liver progenitor cells; Authors - Wen-Ming Liu

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